Coding

Part:BBa_K4129103:Design

Designed by: Magnus Haahr   Group: iGEM22_DTU-Denmark   (2022-10-09)

The synthetic transcription factor, FunsTF05 (LexA-LL-Hmox1-VP16-SV40)

The following is the DNA sequcence of FunsTF05


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 945
    Illegal BamHI site found at 607
    Illegal XhoI site found at 800
    Illegal XhoI site found at 1237
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Design Notes

FunsTF05 consists of modules from LexA, Hmox1 and VP16, and these modules can individually be exchanged with other domains. The exchange of modules follow a standardised USER assembly.

Source

FunsTF05 was de novo synthesised as a gene block from IDT.

References

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Ottoz DS, Rudolf F, Stelling J. Inducible, tightly regulated and growth condition-independent transcription factor in Saccharomyces cerevisiae. Nucleic Acids Res. 2014;42(17):e130. doi: 10.1093/nar/gku616. Epub 2014 Jul 17. PMID: 25034689; PMCID: PMC4176152.

Radman M. SOS repair hypothesis: phenomenology of an inducible DNA repair which is accompanied by mutagenesis. Basic Life Sci. 1975;5A:355-67. doi: 10.1007/978-1-4684-2895-7_48. PMID: 1103845. Santhakumar P, Prathap L, Roy A, Jayaraman S, Jeevitha M. Molecular docking analysis of furfural and isoginkgetin with heme oxygenase I and PPARγ. Bioinformation. 2021 Feb 28;17(2):356-362. doi: 10.6026/97320630017356. PMID: 34234396; PMCID: PMC8225605.

Rantasalo A, Landowski CP, Kuivanen J, Korppoo A, Reuter L, Koivistoinen O, Valkonen M, Penttilä M, Jäntti J, Mojzita D. A universal gene expression system for fungi. Nucleic Acids Res. 2018 Oct 12;46(18):e111. doi: 10.1093/nar/gky558. PMID: 29924368; PMCID: PMC6182139.

Tenhunen R, Marver HS, Schmid R. Microsomal heme oxygenase. Characterization of the enzyme. J Biol Chem. 1969 Dec 10;244(23):6388-94. PMID: 4390967.